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expression vector phis17  (New England Biolabs)


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    New England Biolabs expression vector phis17
    Expression Vector Phis17, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/expression+vector+phis17/pmc04793087__mmc2-182-4-10
    Average 86 stars, based on 1 article reviews
    expression vector phis17 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1
    Article Snippet: .. These were cloned into expression vector pHis17 using Gibson assembly (New England Biolabs, MA), adding the affinity purification tag LHHHHHH at the C-termini. .. For overexpression, C41(DE3) cells (Lucigen, WI) were transformed with the resulting vectors and grown in 2xTY media and induced with 1 mM IPTG at 16 ̊C overnight.

    Expressing:

    Article Title: Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1
    Article Snippet: .. These were cloned into expression vector pHis17 using Gibson assembly (New England Biolabs, MA), adding the affinity purification tag LHHHHHH at the C-termini. .. For overexpression, C41(DE3) cells (Lucigen, WI) were transformed with the resulting vectors and grown in 2xTY media and induced with 1 mM IPTG at 16 ̊C overnight.

    Affinity Purification:

    Article Title: Crystal Structure of the Cohesin Gatekeeper Pds5 and in Complex with Kleisin Scc1
    Article Snippet: .. These were cloned into expression vector pHis17 using Gibson assembly (New England Biolabs, MA), adding the affinity purification tag LHHHHHH at the C-termini. .. For overexpression, C41(DE3) cells (Lucigen, WI) were transformed with the resulting vectors and grown in 2xTY media and induced with 1 mM IPTG at 16 ̊C overnight.



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